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Thermal Cycler
Maximum ramp rate, °C/sec
CFX Opus 96 System: 5
CFX Opus 384 System: 2.5
CFX Opus Deepwell System: 2.5
Average ramp rate, °C/sec
CFX Opus 96 System: 3.3
CFX Opus 384 System: 2
CFX Opus Deepwell System: 2
Heating and cooling method
Peltier
Lid, °C
30–110
Temperature
Range, °C
4–100
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
CFX Opus 96 and 384 Systems:
±0.3 well-to-well after 10 sec of arrival at target
CFX Opus Deepwell System:
±0.4 well-to-well after 10 sec of arrival at target
Thermal Cycler
Maximum ramp rate, °C/sec: 5
Average ramp rate, °C/sec: 3.3
Lid, °C : 30-1103.3
Temperature
Accuracy, °C : ±0.2 of programmed target at 90°C
Uniformity, °C : ±0.3 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C : 30–100
| Sample block type | Fixed |
| Dimensions (W x D x H), cm/in | 28 x 50 x 26 / 11 x 20 x 10 |
| Power | 100-240 V, 50/60 Hz, 850 W max |
| Electrical approvals | IEC, CE |
| Memory | 4.6GB; 100,000 files, which include protocols and run reports |
| Noise level | 50-70 dB at steady state |
| Touch Screen | 8 in. LCD |
| Programming options | Step-based graphical and automatic |
| Security features for regulated environments | Password protection and secure user mode |
| Reporting | Exportable run logs and system logs |
| Temperature control modes | Calculated and block |
| Onboard operating system | Linux |
| Communications | WiFi, BR.io, USB A 2.0, Ethernet |
| Heating and cooling method | Peltier |
| Lid heating | 30–110°C |
| Temperature range | 4–100°C |
| Thermal accuracy | +/-0.2°C of programmed target at 90°C |
| Thermal uniformity | +/- 0.4°C well-to-well within 10 sec of arrival at 90°C |
System-Specific Specifications
 | | | |
|
|---|---|---|---|---|
96-Well | Deepwell | 48/48-Well | 384-Well |
|
| Sample block type | Automated | Automated | Nonautomated | Automated |
| Weight | 15 kg (33 Ib) | 15 kg (33 Ib) | 14 kg (31 Ib) | 14 kg (31 Ib) |
| Sample capacity | 96 x 0.2 ml tubes or 1 x 96-well plate | 96 x 0.2 ml tubes, 48 x 0.5 ml tubes, or 1 x 96-well plate | 2 x 48 x 0.2 ml tubes or 2 x 48-well plates | 1 x 384-well plate |
| Sample nolume | 1-50 µl (10-50 µl recommended) | 1-50 µl (10-125 µl recommended) | 1-50 µl (10-50 µl recommended) | 1-30 µl (3-20 µl recommended) |
| Maximum ramp rate | 5°C/sec | 2.5°C/sec | 4°C/sec | 2.5°C/sec |
| Average ramp rate | 3.3°C/sec | 2°C/sec | 3°C/sec | 2°C/sec |
| (Gradient) Operational range | 30-100°C | 30-100°C | 30-100°C | 30-100°C |
| (Gradient) Programmable span | 8 rows, 1-24°C | 8 rows, 1-24°C | 8 rows, 1-24°C | 16 rows, 1-24°C |
Sample capacity
96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate
Maximum ramp rate, °C/sec
4
Average ramp rate, °C/sec
2.5
Temperature range
4–100°C
Temperature accuracy
±0.5°C of programmed target
Temperature uniformity
±0.5°C well-to-well within 30 sec of arrival at target temperature
Input power
100–150 VAC, 50–60 Hz; 220–240 VAC, 50–60 Hz; 700 W maximum
Display
5.7'' VGA color touch screen
